rabbit anti rat myo d polyclonal antibody  (Bioss)

Journal: Journal of Thoracic Disease

Article Title: Hepatocyte growth factor alleviates alcoholic cardiomyopathy through the Nrf2 pathway—a focus on iron metabolism

doi: 10.21037/jtd-2025-1111

Figure Lengend Snippet: Effects of HGF on proteins involved in iron metabolism. (A) Hepcidin mRNA expression was raised by ethanol stimulation and decreased by HGF treatment; (B,C) alterations in Fpn1, DMT1 and FTH1 caused by Nfe2l2 knockdown after HGF pretreatment detected by western blotting under ethanol exposure. **, P<0.01; ns, P>0.05. DMT1, divalent metal-ion transporter 1; Fpn1, ferroportin; FTH1, ferritin heavy chain 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HGF, hepatocyte growth factor; PBS, phosphate buffered saline; scramble, randomly interrupted sequence; siRNA, small interfering RNA.

Article Snippet: Recombinant rat HGF protein (P1779) was obtained from FineTest (Wuhan, China); rabbit polyclonal anti-rat Fpn1 (26601-1-AP), rabbit polyclonal anti-rat DMT1 (20507-1-AP), goat anti-mouse or anti-rabbit secondary antibodies and mouse monoclonal anti-β-actin from Proteintech (Wuhan, China); rabbit polyclonal anti-rat ferritin heavy chain (FTH1) (PA5-27500) from Thermofisher (Waltham, USA).

Techniques: Expressing, Knockdown, Western Blot, Saline, Sequencing, Small Interfering RNA

Journal: Journal of Thoracic Disease

Article Title: Hepatocyte growth factor alleviates alcoholic cardiomyopathy through the Nrf2 pathway—a focus on iron metabolism

doi: 10.21037/jtd-2025-1111

Figure Lengend Snippet: Effects of HGF on proteins involved in iron metabolism. (A) Hepcidin mRNA expression was raised by ethanol stimulation and decreased by HGF treatment; (B,C) alterations in Fpn1, DMT1 and FTH1 caused by Nfe2l2 knockdown after HGF pretreatment detected by western blotting under ethanol exposure. **, P<0.01; ns, P>0.05. DMT1, divalent metal-ion transporter 1; Fpn1, ferroportin; FTH1, ferritin heavy chain 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HGF, hepatocyte growth factor; PBS, phosphate buffered saline; scramble, randomly interrupted sequence; siRNA, small interfering RNA.

Article Snippet: Recombinant rat HGF protein (P1779) was obtained from FineTest (Wuhan, China); rabbit polyclonal anti-rat Fpn1 (26601-1-AP), rabbit polyclonal anti-rat DMT1 (20507-1-AP), goat anti-mouse or anti-rabbit secondary antibodies and mouse monoclonal anti-β-actin from Proteintech (Wuhan, China); rabbit polyclonal anti-rat ferritin heavy chain (FTH1) (PA5-27500) from Thermofisher (Waltham, USA).

Techniques: Expressing, Knockdown, Western Blot, Saline, Sequencing, Small Interfering RNA

Journal: Bone Reports

Article Title: Partial cementocyte ablation does not reduce cellular cementum apposition in a mouse model of molar super-eruption

doi: 10.1016/j.bonr.2025.101873

Figure Lengend Snippet: Effects of diphtheria toxin ablation of Dmp1 -expressing cells on the mouse skeleton. (A) Schematic illustrating study design including the timeline of 0.5 mg/kg diphtheria toxin (DT) administration and first maxillary molar extraction in iDTR fl/fl (CTR) and Dmp1-Cre/iDTR fl/fl (Dmp1-DTR) mice. (B) Body weight analysis reveals no significant changes between the two groups during the experiment ( n = 3–6/group). (C) Representative 3D microCT renderings showing cortical bone (taupe), bone marrow space (amber), and pore spaces (red-brown), reveal increased porosity in Dmp1-DTR vs. CTR femurs as a result of DT administration. (D) Measurements of cortical bone porosity are increased in Dmp1-DTR vs. CTR mice, including cortical porosity (Ct.Po), pore number (Po.N), and pore volume (Po.V) ( n = 4–6/group). *** p < 0.001; **** p < 0.0001.

Article Snippet: Primary antibodies included: polyclonal rabbit anti-mouse osteopontin (OPN) IgG (LF-175, courtesy of Dr. Larry Fisher, NIDCR/NIH, Bethesda, MD, USA) ( ; ); and polyclonal rabbit anti-rat dentin matrix protein 1 (DMP1) IgG (M176; Takara Bio USA Inc., San Jose, CA, USA) ( ).

Techniques: Expressing, Extraction

Journal: Bone Reports

Article Title: Partial cementocyte ablation does not reduce cellular cementum apposition in a mouse model of molar super-eruption

doi: 10.1016/j.bonr.2025.101873

Figure Lengend Snippet: Osteocyte and cementocyte ablation by diphtheria toxin. We assessed the effects of DT on osteocytes of the alveolar bone (AB) and basal bone (BB) and cementocytes of the cellular cementum (CC). By H&E staining, empty lacunae (black arrowheads in panel B) and those containing pycnotic nuclei were counted and normalized to total cells in the regions. By TUNEL staining, positive (green) cells were counted and normalized to total cells (blue) in the regions. Compared to (A) CTR mice, which show few empty, pycnotic, or apoptotic cells, (B) Dmp1-DTR mice show increased numbers of dying and dead cells. (C) H&E staining indicates 40–50 % of osteocytes and cementocytes are affected by DT in Dmp1-DTR mice ( n = 2/group). (D) TUNEL staining shows 50–80 % of osteocytes and cementocytes undergoing apoptosis in Dmp1-DTR mice (n = 2/group). DE = dentin; IN = incisor.

Article Snippet: Primary antibodies included: polyclonal rabbit anti-mouse osteopontin (OPN) IgG (LF-175, courtesy of Dr. Larry Fisher, NIDCR/NIH, Bethesda, MD, USA) ( ; ); and polyclonal rabbit anti-rat dentin matrix protein 1 (DMP1) IgG (M176; Takara Bio USA Inc., San Jose, CA, USA) ( ).

Techniques: Staining, TUNEL Assay

Journal: Bone Reports

Article Title: Partial cementocyte ablation does not reduce cellular cementum apposition in a mouse model of molar super-eruption

doi: 10.1016/j.bonr.2025.101873

Figure Lengend Snippet: Diphtheria toxin ablation of Dmp1 -expressing cells reduces alveolar bone density. (A, B) Representative 2D and 3D microCT images of the mandibular first molar (M1) from CTR and Dmp1-DTR mice at 2 months. Yellow asterisks in 2D images indicate increased porosity in alveolar bone (AB) of Dmp1-DTR mice. (C) Enamel and dentin volumes and mineral densities are unchanged by DT administration, while AB volume is increased and density is decreased in Dmp1-DTR vs. CTR mice (n = 4–6/group). * p < 0.05; ** p < 0.01; ns, not significant.

Article Snippet: Primary antibodies included: polyclonal rabbit anti-mouse osteopontin (OPN) IgG (LF-175, courtesy of Dr. Larry Fisher, NIDCR/NIH, Bethesda, MD, USA) ( ; ); and polyclonal rabbit anti-rat dentin matrix protein 1 (DMP1) IgG (M176; Takara Bio USA Inc., San Jose, CA, USA) ( ).

Techniques: Expressing

Journal: Bone Reports

Article Title: Partial cementocyte ablation does not reduce cellular cementum apposition in a mouse model of molar super-eruption

doi: 10.1016/j.bonr.2025.101873

Figure Lengend Snippet: Diphtheria toxin ablation of Dmp1 -expressing cells did not affect cellular cementum under experimentally-induced apposition. (A) 3D and 2D images of the mandibular first molar (M1) from Dmp1-Cre/iDTR fl/fl (cKO) and iDTR fl/fl (CTR) mice at 3 weeks after maxillary M1 extraction. Cellular cementum (CC) is shown in yellow and CC pores (approximating cementocyte lacunae) are shown in red. (B) Quantitative analysis of cellular cementum (CC) volume, CC pore volume, CC mineral density (BMD), and CC tissue mineral density (TMD) show no differences between experimental groups. Ns = not significant.

Article Snippet: Primary antibodies included: polyclonal rabbit anti-mouse osteopontin (OPN) IgG (LF-175, courtesy of Dr. Larry Fisher, NIDCR/NIH, Bethesda, MD, USA) ( ; ); and polyclonal rabbit anti-rat dentin matrix protein 1 (DMP1) IgG (M176; Takara Bio USA Inc., San Jose, CA, USA) ( ).

Techniques: Expressing, Extraction

Journal: Bone Reports

Article Title: Partial cementocyte ablation does not reduce cellular cementum apposition in a mouse model of molar super-eruption

doi: 10.1016/j.bonr.2025.101873

Figure Lengend Snippet: Extracellular matrix composition of cellular cementum after experimentally-induced apposition. (A, B) Immunostaining shows similar distributions of osteopontin (OPN) (red-brown dye, indicated by blue stars) between experimental groups in cellular cementum (CC), periodontal ligament (PDL), and alveolar bone (AB). (C, D) Immunostaining for dentin matrix protein 1 (DMP1) shows reduced localization in the Dmp1-DTR vs. CTR group, with reduced labeling of cementocyte (Ccy) lacunocanalicular networks (black arrowheads in high magnification insets). (E–H) Picrosirius red (PR) staining viewed under polarized light shows collagen orientation. The same section of a CTR mouse is shown in panels E–F and of a Dmp1-DTR mouse in panels G–H, with the polarizer positioned to maximize signal in PDL for panels E and G and CC for panels F and H. In panels E and G, PDL shows highly oriented and tightly packed yellow hued fibers (white arrowheads) that enter the cervical and mid-root as red-colored Sharpey's fibers. In panels F and H, CC collagen is less organized, with most fibers oriented randomly, but some organized fibers vertically entering from apical PDL (white arrowheads). No differences in collagen orientation are observed between experiment groups. DE = dentin.

Article Snippet: Primary antibodies included: polyclonal rabbit anti-mouse osteopontin (OPN) IgG (LF-175, courtesy of Dr. Larry Fisher, NIDCR/NIH, Bethesda, MD, USA) ( ; ); and polyclonal rabbit anti-rat dentin matrix protein 1 (DMP1) IgG (M176; Takara Bio USA Inc., San Jose, CA, USA) ( ).

Techniques: Immunostaining, Labeling, Staining